rabbit polyclonal antibodies against α sma  (Proteintech)

Journal: Microbiology Spectrum

Article Title: A novel chronic hepatitis B mouse model with immune activation and liver fibrosis

doi: 10.1128/spectrum.02513-24

Figure Lengend Snippet: Progressed liver inflammation, injury, and fibrosis caused by injections of PS in pAAV-HBV 1.2 BALB/c mice. Levels of collagen deposition were appraised by ( A, B ) Sirius red (SR) staining for liver sections, and by ( C ) the quantification of hepatic hydroxyproline (Hyp, n = 6); for the representative images of SR staining, 200×, scale bar = 100 µm; the top-right corner, 100×; scale bar = 200 µm. Hepatic α-SMA protein levels were analyzed by ( D, E ) IHC ( n = 4) and by ( F, G ) WB ( n = 3); for the representative IHC micrographs, the upper panels, 100×; scale bar = 200 µm; the lower panels, 200×; scale bar = 100 µm. The pathological changes in the liver were observed through ( H ) H&E staining (12 wpi), and ( I ) the liver inflammation grades and ( J ) the fibrosis stages were, respectively, assessed by the Scheuer system and Ishak scoring system, respectively. For the representative images of H&E staining, portal area: the upper panels, 100×; scale bar = 200 µm; the lower panels, 200×; scale bar = 100 µm; interstitium: 50×; scale bar = 400 µm. All data are presented as mean ± SEM. * P < 0.05, ** P < 0.01.

Article Snippet: Liver sections (3 μm) were incubated in 3% hydrogen peroxide for 30 min followed by a PBS wash. Then, the sections were blocked with 3% fetal bovine serum at room temperature following heat-mediated antigen retrieval and incubated successively with primary antibodies against α-SMA rabbit polyclonal antibody (1:800; Proteintech Group, Inc., Wuhan, China), HBcAg (1:50; Gene Tech [Shanghai] Company Limited), or PD-L1 (1:100; HUABIO, Huaan Biotechnology Co., Ltd., Hangzhou, China), at 4°C overnight, and secondary antibody (1:300; Thermo Fisher Scientific [China] Co., Ltd.) at 37°C for 45 min, and then treated with a diaminobenzidine chromogenic substrate, counterstained with hematoxylin, sealed, and scanned with an Aperio Versa scanner (Leica, Germany).

Techniques: Staining

Journal: Microbiology Spectrum

Article Title: A novel chronic hepatitis B mouse model with immune activation and liver fibrosis

doi: 10.1128/spectrum.02513-24

Figure Lengend Snippet: Attenuation of liver fibrotic formation in model mice via HBV replication inhibition by entecavir. ( A ) Experiment scheme showing ETV treatment for 6 weeks from 6 wpi. ( B ) HBV DNA titers based on qPCR after ETV treatment for 2, 4, and 6 weeks, n = 6. ( C ) Representative micrographs (IHC) showing the HBc proteins and ( D ) the statistical analysis. ( E ) Representative images of H&E-stained liver sections showing pathological changes after ETV treatment for 6 weeks (100×, scale bar = 200 µm). ( F ) Representative images of SR-stained liver sections (200×, scale bar = 100 µm; the top-right corner, 100×; scale bar = 200 µm) and ( G ) the statistical analysis ( n = 6). ( H ) Representative micrographs (IHC) showing α-SMA proteins and ( I ) the statistical analysis ( n = 4). All data are presented as mean ± SEM. * P < 0.05, ** P < 0.01.

Article Snippet: Liver sections (3 μm) were incubated in 3% hydrogen peroxide for 30 min followed by a PBS wash. Then, the sections were blocked with 3% fetal bovine serum at room temperature following heat-mediated antigen retrieval and incubated successively with primary antibodies against α-SMA rabbit polyclonal antibody (1:800; Proteintech Group, Inc., Wuhan, China), HBcAg (1:50; Gene Tech [Shanghai] Company Limited), or PD-L1 (1:100; HUABIO, Huaan Biotechnology Co., Ltd., Hangzhou, China), at 4°C overnight, and secondary antibody (1:300; Thermo Fisher Scientific [China] Co., Ltd.) at 37°C for 45 min, and then treated with a diaminobenzidine chromogenic substrate, counterstained with hematoxylin, sealed, and scanned with an Aperio Versa scanner (Leica, Germany).

Techniques: Inhibition, Staining

Journal: Frontiers in Pharmacology

Article Title: Identifying potential drugs for treating Cardiovascular-kidney metabolic syndrome via reverse network pharmacology

doi: 10.3389/fphar.2025.1627236

Figure Lengend Snippet: Construction of CKM mouse model: Renal Part: (A) Serum indicators of uric acid, creatinine, and urea nitrogen, (B) quantification of kidney weight, (C) typical images evaluated using two staining methods for Renal pathological changes: In the HFD group, glomeruli are normal in size and shape with clear boundaries. The glomerular capsule is very distinct. In the CKM group, glomeruli are deformed, enlarged, filled with blood cells, and capillaries are dilated. There is fat deposition in the renal tubular interstitium (black arrow), and inflammatory cell deposition (red arrow). MASSON staining shows significant fibrosis in the glomeruli and renal interstitium of the CKM group (blue area). (D) Renal injury score based on HE staining. (E) , semi-quantitative analysis of MASSON staining of the kidneys, including fibrosis area. (F) , Expression Analysis of fibrosis index:Representative Western blot images showing the protein expression levels of COL3a1, TGFβ, α-SMA and β-actin. (G) : Quantification of Western blot results. (mean ± SD, n = 5, P < 0.05 was statistically significant, ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05).

Article Snippet: Rabbit monoclonal antibodies against α-SMA (Proteintech Group, China; #14395-1-AP, 1:2000), TGFβ(ABclonal, China; #A23262, 1:2000), Col1a1 (ABclonal, China; #A24112, 1:1,000), and β-actin (ABclonal, China; #AC026, 1:500) were added and incubated overnight at 4°C.

Techniques: Staining, Expressing, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Identifying potential drugs for treating Cardiovascular-kidney metabolic syndrome via reverse network pharmacology

doi: 10.3389/fphar.2025.1627236

Figure Lengend Snippet: The effects of BBR on kidney function and fibrosis in CKM mice: (A) Serum indicators such as uric acid, creatinine, and blood urea nitrogen were significantly reduced in the low-dose BBR group compared to the high-dose group; (B) Typical images of Renal pathological changes evaluated using two staining methods: CKM + Vehicle shows glomerular deformation, increased volume, and engorged with blood cells, along with dilated glomerular capillaries. There is fat deposition in the renal tubular interstitium (black arrow), and inflammatory cell deposition (red arrow). MASSON staining reveals significant fibrosis in the glomeruli and renal interstitium of the CKM group (blue area). The CKM + BBR group shows better recovery compared to the CKM group; (C) Quantification of kidney weight; (D) Renal injury score based on HE staining. (E) , Semi-quantitative analysis of MASSON staining in the kidneys to determine the area of fibrosis. (F) , Expression Analysis of Relative mRNA expression levels of fibrosis markers:Representative Western blot images showing the protein expression levels of COL3a1, TGFβ, α-SMA and β-actin. (G) : Quantification of Western blot results. (H) : Vimentnt, Col1a1, MMP9, Acta2. (mean ± SD, n = 5, P < 0.05 was statistically significant, ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05).

Article Snippet: Rabbit monoclonal antibodies against α-SMA (Proteintech Group, China; #14395-1-AP, 1:2000), TGFβ(ABclonal, China; #A23262, 1:2000), Col1a1 (ABclonal, China; #A24112, 1:1,000), and β-actin (ABclonal, China; #AC026, 1:500) were added and incubated overnight at 4°C.

Techniques: Staining, Expressing, Western Blot

Journal: Scientific reports

Article Title: Polystyrene microplastics induce liver fibrosis and lipid deposition in mice through three hub genes revealed by the RNA-seq.

doi: 10.1038/s41598-025-86810-5

Figure Lengend Snippet: Fig. 2. PS-NMPs exposure promotes liver fibrosis. (A) Representative images of MTS of liver tissue and (B) semi-quantitative results of tubulointerstitial fibrosis. Scale bar, 100 mm. (C) The mRNA level of α-sma was analyzed by qRT-PCR and normalized by β-actin (n = 6). (D) α-SMA protein level was analyzed by western blotting and normalized by β-actin (n = 5). (E) Representative images of IHC staining for α-SMA and (F) semiquantification of α-SMA based on IHC staining (n = 8). Scale bar, 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After blocking with TBS containing 5% skim milk for 1 h at room temperature, the PVDF membrane was incubated with rabbit polyclonal antibody against α-SMA (Proteintech, #14395-1-AP, 1:1000) and mouse polyclonal antibody against IL-1β (Proteintech, #66737-1-lg, 1:1000).

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemistry